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Introgression of novel genetic variation in Brassica carinata

机译:甘蓝型油菜新遗传变异的基因渗入

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Brassica carinata (BBCC, 2n=34) has still to emerge as a major oilseed crop owing to poor agronomic attriutes like long duration, low oil content and hard to thresh siliqua. Interspecific hybridization followed by selection in selfed and backcross progenies was employed to introgress useful variability into B. carinata cv PC 5 from two related digenomics viz. B. napus (AACC, 2n=38) and B. juncea (AABB, 2n=36). Hybridity of B. napus×B. carinata and B. juncea×B. carinata crosses was confirmed through cytological and molecular analysis. Forty six F3, BC2 and BC1 F2 B. carinata type prrogenies werre identified on the basis of morphology and high pollen grain stainability. Almost all these progenies had high bivalent frequency (16-17 Ⅱ) indicating their relative genomic stability. Morphological and seed quality evaluation of these progenies indicated significant variation for key economic traits. Individual plants / progenies with low erucic acid ( 11.8% in BNC 2 vs. 45.5% in PC 5), high oil content (41.5% in BJC1 vs. 34.5%in PC 5), early maturity (157 days vs. 168 days in PC 5) and short stature (140 cm vs. 231 cm in PC 5) were selected. Diversity analysis (UPGMA) on the basis of morphological variation grouped 42 of the 46 progenies,with B. carinata cv. PC5 at a taxonomic distance of 1.09 inferring enlarged genetic base. At molecular (RAPD) level also 17 of the selected 21 progenies grouped with B. carinata cv. PC5 at a similarity coefficient of 0.63, confirming broadly the clustering based on morphological diversity. The use of identified variability in breeding programmes is likely to help in the development of early maturing, canola quality B. carinata cultivars having high oil content and desired morphotype. Increasing oil content to 41.5 per cent was a significant achievement of the study.
机译:由于不良的农艺性状,例如持续时间长,含油量低和难以脱粒的硅,芸苔(BBCC,2n = 34)仍是主要的油料作物。种间杂交,然后在自交和回交后代中进行选择,被用于从两个相关的双基因组学中将有用的变异性渗入到卡氏芽孢杆菌cv PC 5中。 B.napus(AACC,2n = 38)和B.juncea(AABB,2n = 36)。甘蓝型油菜×B的杂交。卡里纳塔和芥菜× Carinata杂交通过细胞学和分子分析得到证实。根据形态学和高花粉粒可染性鉴定了46种F3,BC2和BC1 F2肉芽孢杆菌致病菌。这些后代几乎都具有较高的二价频率(16-17Ⅱ),表明它们的相对基因组稳定性。这些后代的形态和种子质量评估表明关键经济性状存在显着差异。低芥酸(BNC 2中为11.8%,PC 5中为45.5%),高油含量(BJC1中为41.5%,而PC 5中为34.5%),早熟(157天对168天)的单个植物/后代选择PC 5)和矮个子(140 cm,而PC 5中为231 cm)。基于形态变异的多样性分析(UPGMA)将46个后代中的42个与卡氏芽孢杆菌(B. carinata cv。在分类距离为1.09处的PC5推断出扩大的遗传基础。在分子水平(RAPD),所选的21个后代中的17个也与卡氏芽孢杆菌(B. carinata cv)分组。 PC5的相似系数为0.63,从而在很大程度上确认了基于形态多样性的聚类。在育种程序中使用已确定的变异性可能有助于开发具有高含油量和所需形态的油菜品质的油菜双歧杆菌。将含油量提高到41.5%是这项研究的一项重大成就。

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