Detecting system based on framing camera for suspension array

摘要

High speed imaging technology has been applied on biomedical research for a long history. Suspension array technology is a new generation of biochip, which was widely used in fields of life science and analytical chemistry, and was developed quickly. This study present a detecting system based on framing camera for suspension array. In suspension array microspheres were used as the carrier of bio-probes and microchannels were used as analyzing platform. By pre-dyeing of fluorophores in microbeads, the addressing of microbeads was implemented by optical coden. Bio-probes attached to microbeads were distinguished by intensity of fluorescence. Suspension array was usually detected with flow cytometry serially, which was slow relatively. Then a 2D parallel measurement system based on framing camera for suspension array was established in order to increase the measurement speed. Liquid sample containing microsphere was injected into microchannel by a 100ul syringe connected by a capillary. Microspheres flowing in the microchannel form a 2D layer, which was illuminated freezingly by a pulsed Xenon lamp and imaged by a microscopy objective in parallel. The microfluidic channel was designed and fabricated, which was a rectangle microchannel of 1mm×50um in cross-section. The image was captured by CCD and transmitted into computer by frame grabber. Image was processed to distinguish microspheres extract information from the background. Thus area measurement of suspension array in microchannel was realized. Compared with flow cytometry, this technology increased analyzing rate greatly, which could be thousands of microspheres per second.