Humic substances, typical example of dissolved organic matters found in nature, largely exist in leachate as well as sedimentary layers of lakes & marshes, organic sewage sludge and waste water from livestock. Dissolved humic substances generally exist as colloidal states with yellow to black colours causing chromaticity in water. These non-biodegradable substances induce many problems during water treatment process such as disturbance of coagulation and decrease in absorption efficiency of activated carbon. In this study, an input of high-concentrated organic matters containing high molecule humic substances was increased in different phases to carry on biological treatment of leachate followed by coagulation treatment. Then, removal characteristics of leachate treatment in the each input phases were examined. The results showed that the amount of humic substances in the biological treatment process rises; the more increase in input of high molecule humic substances the more accompanied with an increase of most molecule substances. It is also shown that most small molecules under 0. 5 kDa with high biodegradability were removed in the biological treatment process. Non-degradable high molecules humic substances over 30 kDa, which remained in the biological treatment process, were more effectively removed in the coagulation treatment process with an improved water quality compared with those in the process without input of high molecule substances.
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