BENZENE AND AROMATIC HYDROCARBONS 'POOL' DETERMINATION IN UNLEADED FUELS, BY A WHOLE CELL BIOSENSOR WORKING IN HYDROPHOBIC ORGANIC SOLVENT

摘要

A biosensor has been developed, based on immobilised Pseudomonas putida cells, operating in n-hexane suitable for determining benzene in hydrophobic matrices in which benzene was contained. The biosensor may be also used in the initial screening, of the pool of aromatic hydrocarbons contained in unleaded fuel, operating directly in n-hexane and using a relatively cheap and simple instrumental set-up.