Enzymatic degradation of carbaryl by pseudomonas cepacia and isolation of a plasmid responsible for drgradation

摘要

The increased sue of various types of pesticides in agriculature has led to much greater emphasis on the possibility of serious environmental contamination arising from their use. Although pesticides are deliberately designed to alter the balance of ecology, that is to eliminate or restrict undesirable species in favour of species considered desirable for man's continued existence but the ubiquotous nature of so many biological and biochemical processes makes in unlikely that even highly specific pesticides will not affect some other biosystems. Some of these chemical persist for longer times in soil and water, however, these do get degraded naturally by a number of means. But it is needed that these pesticides be degraded in an accelerated manner which can be attained by biological means using acclimated microorganism. In the present study, a soil bacterium, paeudomonas cepacia was isolated which efficiently degraded carbaryl in laboratory and soil environment. It could degrade 70