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Comparative study of the lignocellulolytic enzymes produced by wood-rotting fungi during eucalyptus grandis decay

机译:木腐真菌在桉树腐烂过程中产生的木质纤维素分解酶的比较研究

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The pattern of lignocellulolytic enzymes produced by several wood rot fungi when cultured on Eucalyptus grandis wood chips under solid culture conditions was studied. Fungal proteins were extracted at different times during the decay period (90 days) and the hydrolytic and ligninolytic enzymes were determined. The brown-rot fungi produced mainly hydrolytic enzymes during the decay. Oxidative enzymes were not detected on the extracts of these fungi, except for the oxidase activity which was detected in Poria cocos cultures. All white-rot fungi produced hydrolytic and ligninolytic enzymes. Nevertheless, hydrolytic activities were lower than that produced by brwon-rot fungi, except for the #beta# -glucosidase levels. The highest levels of ligninolytic activities were obtained in Trametes versicolor cultures. These enzymatic activities decreased from initial (30 days) to the final (90 days) decays stages.
机译:研究了在固态培养条件下,在巨桉木片上培养几种腐烂真菌产生的木质纤维素分解酶的模式。在腐烂期(90天)的不同时间提取真菌蛋白,并测定水解酶和木质素酶。褐腐真菌在腐烂过程中主要产生水解酶。除了在Por培养物中检测到的氧化酶活性外,在这些真菌的提取物中未检测到氧化酶。所有的白腐真菌都产生水解和木质素分解酶。然而,除了#β#-葡糖苷酶水平外,水解活性低于Brwon-rot真菌产生的水解活性。在Trametes versicolor培养物中获得了最高水平的木质素分解活性。这些酶活性从最初(30天)到最终(90天)衰变阶段减少。

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