Preparation of affinity membrane captridges for the removal of endotoxins from aqueous solutions

摘要

Affinity membrane cartridges with histidine and polymyxin B immobilized as affinity ligands were used for the removal of endotoxins from aqueous solutions. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking. and was used as matrix for the immobilization of affinity ligand. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine or polymyxin B were immobilized. Membrane cartridges were also prepared, which can be usedto remove pyrogen from aqueous solutions. By a cartridge with 40 sheets of affinity membrane, the endotoxin content in solution can be reduced to a minimum of 0.04 EU/mL.