Production of poly-beta-hydroxybutyrate and measurmenet of relevant enzyme activities in methylosinus trichosporium OB3b

摘要

Production of poly-beta-hydroxybutyric acid (PHB) and three key enzymes related to its synthesis, beta-ketothiolase (beta-KT), NADPH-dependent acetoacetyl-CoA reductase (AAR), and citrate synthase (CS) were studied in an obligatory methanotroph Methylosinus trichosporium OB3b. When cultivated in a two-stage mdoe, the initial growth-stage in a complete medium and the followign production-stage in a nitrate-deficient medium, M. trichosporium OB3b showed a significant difference in the cellular PHB level depending on the medium Cu availability during its growth. When Cu was available, the level was more than 50