In spectroscopic investigations of biological samples the problem of separation and quantitative evaluation of spectra lines connected with various molecules is the most important one. The situation is especially significant for in vivo investigations. This paper presents the results of fluorescence spectra analysis of some photosensitizers measured in various tissues both in experimental and clinic conditions and autofluorescence spectra analysis of some human tissues. It is shown, that technique proposed allows one to evaluate the component concentrations including the in vivo measurements.
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