A Study on the Cell Patterning Method using Poly(ethylene terephthalate)Modification for Electron Microbeam Experiments

摘要

It is recognized that a microbeam is powerful system to understand the interaction of ionizing radiation with cells. Especially,electron microbeam system is useful to investigate the effect of low-LET radiation for cells. When the electron microbeamirradiates an individual cell, however, spatial effect can be arisen from the electron scattering around the target cell. So, it isnecessary to develop a new cell patterning method suitable for microbeam experiments.We use a 2.5 mm poly (ethylene terephthalate) film as a cell culture ground, and the film is highly crystalline andhydrophobic in nature. It is needed to develop a hydrophobic region to adhere the cells and a hydrophilic region to repel thecells for the patterned surface on PET films. So we modify the PET film through two-stage modification. As a first stage wecover thin hydrophilic layer onto entire PET films. One of the most common ways to modify hydrophobisity is graftinghydrophilic monomers on it. Monomers can be grafted onto PET films either by radiation or chemical means. In this research,the chemical initiation method is used for grafting.Graft co-polymerization of N-isopropylacrylamide (NIPAAm) on PET films using benzoyl peroxide (Bz2O2) initiator hasbeen studied. Variation of graft yield with the monomer concentration, initiator concentration, polymerization temperature, andreaction time are also investigated.