White Matter Lesions and Cerebral Palsy Model of Neonatal Rat Brain with 3-nitropropionic Acid

摘要

A model for periventricular leukomalacia (PVL) by intracerebral injection of 3-nitropropionic acid (3-NPA) and explore realistic model for concerned studies was established. 64 of Sprague Dawley rat pups of both sexes at the age of postnatal day 5 (P5) were randomized into two groups: NPA group (n=32) and PBS group (n=32), and injected the same volume of 3-NPA and PBS with a tip located at the pup's collosum on the left ventricle by stereotaxis instrument, respectively. 24 hours, 48 hours, 72 hours and 9 days after injection, the injections were perfused and then brains were collected. Then ice sections of brains were performed for HE staining, oligodendrocyte O4 and MBP immunostaining. HE staining showed that, in the NPA group, sub-cortical and periventricular white matter rarefaction were observed on P6, P7 and P8, a significant lateral ventricle enlargement was found on P14 compared with the PBS group (P<0.01), and no histological changes in gray matter were observed. O4 staining showed positive cells in the NPA group decreased apparently compared with the PBS group (P<0.01). MBP immunostaining showed the mean optical density value in the NPA group was significantly lower than that in the PBS group (P<0.05). It was found that the model for PVL induced by intracerebral injection of 3-NPA was characterized by damage to the periventricular white matter leaves (PVL), while the cortical and grey matter were intact. The model can be used to study concerned disease as a realistic one.