A Study on DNA Breaks and DNA-protein Crosslinks Induced by Formaldehyde in two Types of Mice Cells

摘要

Objective: Previous studies have confirmed that formaldehyde (FA) is genotoxic and mutagenic to mammalian cells. FA can induce a broad spectrum of genetic effects. This study is undertaken to investigate the direct effect of formaldehyde on two types of mice cells. Methods: The single cell gel electrophoresis (SCGE) and KCl-SDS assay were used to detect the effect of FA in mice lung cells and mast cell line (P815) in vitro. Results: The effects of formaldehyde in the two types of cells were similar. The results showed that liquid formaldehyde induced DNA breakage at 5 μM, 25 μM (P<0.01, compared with control group) and the DNA-protein crosslinks were found at high concentrations (125 μM P<0.05; 625 μM P<0.01) Conclusions: The results showed that formaldehyde caused DNA breaks and crosslinks for different types of mice cells.