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Histological study of subcutaneous fat at NIR laser treatment of the rat skin in vivo

机译：NIR激光体内治疗大鼠皮肤皮下脂肪的组织学研究

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摘要

The goal of this work is to quantify impact of in vivo photochemical treatment using indocyanine green (ICG) or encapsulated ICG and NIR laser irradiation through skin of rat with obesity by the follow up tissue sampling and histochemistry. After 1 hour elapsed since 1-min light exposure samples of rat skin with subcutaneous tissue of thickness of 1.5-2.5 mm were taken by surgery from rats within marked 4-zones of the skin site. For hematoxylin-eosin histological examination of excised tissue samples, fixation was carried out by 10%-formaldehyde solution. For ICG and encapsulated ICG subcutaneous injection and subsequent 1-min diode laser irradiation with power density of 8 W/cm~2, different necrotic regions with lipolysis of subcutaneous fat were observed. The obtained data can be used for safe layer-by-layer laser treatment of obesity and cellulite.
机译：这项工作的目的是通过后续的组织采样和组织化学方法，对使用吲哚花青绿（ICG）或封装的ICG和NIR激光辐照肥胖大鼠的皮肤进行体内光化学治疗的影响进行量化。自1分钟曝光以来经过1小时后，通过手术从皮肤部位的显着4区内的大鼠中取出厚度为1.5-2.5mm的皮下组织的大鼠皮肤样品。为了对切除的组织样品进行苏木精-伊红组织学检查，用10％甲醛溶液固定。对于ICG和封装的ICG皮下注射，随后以8 W / cm〜2的功率密度照射1分钟的二极管激光，观察到不同的坏死区域以及皮下脂肪的脂解作用。所获得的数据可用于肥胖症和橘皮组织的安全逐层激光治疗。

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  • 来源
  • 会议地点 Munich(DE)
  • 作者

    I. Yu. Yanina; Yu. I. Svenskaya; N. A. Navolokin; O. V. Matveeva; A. B. Bucharskaya; G. N. Maslyakova; D. A. Gorin; G. B. Sukhorukov; V. V. Tuchin;

  • 作者单位

    Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russia,Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Saratov State Medical University, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Saratov State Medical University, Russia;

    Saratov State Medical University, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Saratov State Medical University, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Saratov State Medical University, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Nano- and Biomedical Technology Department, Saratov State University, Saratov, Russia;

    Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Queen Mary University of London, UK;

    Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russia,Remote Controlled Theranostic Systems Lab, Education and research Institute of Nanostructures and Biosystems, Saratov, Russia,Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Russia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    laser irradiation; photodynamic action; adipose tissue; necrosis; histological analysis;

    机译：激光照射光动力作用脂肪组织;坏死组织学分析;

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