Raman Study of the Effect of LED Light on Grafted Bone Defects

摘要

Benefits of the isolated or combined use light and biomaterials on bone healing have been suggested. Our group has used several models to assess the effects of laser on bone. A Raman spectral analysis on surgical bone defects grafted or not with Hydroxyapatite (HA), treated or not with LED was carried out. 40 rats were divided into 4 groups. On Group I the defect was filled with the clot. On Group Ⅱ, the defect was filled with the HA. On groups Ⅲ the defect was filled with Clot and further irradiated with LED and on group Ⅳ the defects was filled with the HA and further irradiated with LED. LED (λ850 ± l0nm, 150mW, A= 0.5cm~2, 68s, 20 J/cm~2 per session, 140 J/cm~2 per treatment) was applied at 48 h intervals during 15 days. Specimens were taken after 15 and 30 days after surgery and kept on liquid nitrogen, and underwent Raman analysis. For this, the peak of hydroxyapatite (～960 cm~(-1)) was used as marker of bone mineralization. Significant difference was observed at both times (p<0.05). When the biomaterial was used higher peaks were observed. Association with LED further improved the intensity. Conclusion: It is concluded that LED light improved the effect of the HA.