SSR/CISP MARKER ANALYSIS FOR GENETIC RELATIONSHIP

摘要

Zoysia grasses are well adapted to warm climates and the transition zone in the United States.In this study,the genetic relationship of 11 turfgrass commercial cultivars and 12 experimental genotypes of Zoysia spp.was estimated using 24 Zoysia-specific simple sequence repeat (SSR) and six conserved-intron scanning primer (CISP) markers previously developed for the warm season grasses such as rice,sorghum,and Pennisetum.All 23 Zoysia accessions tested,except ‘Zorro’ and ‘Zeon’,were genetically fingerprinted using 51 allelic amplicons.The SSR markers were able to separate the cultivars ‘Empire’ and ‘El Toro’,which were previously inseparable using AFLP markers.The dendrogram constructed using the UPGMA method indicated that the accessions were classified into three clusters.All cultivars (or genotypes) in cluster I corresponded to cold tolerant individuals,while clusters II and III represented accessions with high or intermediate susceptibility to winter injury,respectively.Overall,accessions bred from either common parents or the same collection sites were categorized in the same cluster.These results demonstrate the effectiveness of Zoysia SSR markers and the transferability of six CISP markers for genetic diversity of these cultivars and experimental genotypes,providing a useful genetic tool for improvement of Zoysia germplasm.