SUGARCANE YELLOW LEAF VIRUS IN THE WORLD COLLECTION OF SUGARCANE AND RELATED GRASSES AT MIAMI, FLORIDA

摘要

THE STATUS of Sugarcane yellow leaf virus (SCYLV) infection was determined in Saccharum clones maintained in field plots at the World Collection of Sugarcane and Related Grasses at the USDA-ARS Subtropical Horticultural Research Station at Miami, Florida to identify putative virus-free and infected clones. Five leaves were collected from each clone from different plants in the 3 m plot. The SCYLV infection status was determined by the tissue blot immunoassay. The incidence of SCYLV infection varied among Saccharum spp. The incidence of SCYLV was 5.6% for 252 S. spontaneum clones, 12.7% for 55 S. barberi clones, 45.9% for 37 S. sinense clones, 60.0% for 55 S. robustum clones and 78.2% for 325 S. officinarum clones evaluated. Of 175 Saccharum hybrid clones 70.9% were infected. Several clones (Bandejarmain Hitan, Chunnee, Co 213, Co 285, POJ 100, POJ 213, and POJ 2878) that were used early in breeding programs were SCYLV infected; whereas clones Kassoer, Co 206, Co 281 and POJ 1499 were putatively virus-free. Both NCo 310 and NCo 376, two widely grown cultivars, were infected. Ratings for the individual clones will be posted on the website: http://www.ars-grin.gov/ars/SoAtlantic /Miamigr/gallry01.htm. The low SCYLV infection found in the S. spontaneum clones suggests this group is putatively the most resistant. The high incidence of SCYLV in the hybrid clones is consistent because a number of clones in their early pedigrees were susceptible.