A new method of quantitative determination of apoptotic parameters in cellular suspensions

摘要

in report a new method of quantitative determination of apoptosis parameters in cellular suspensions using correlation optic methods is considered. The principle of the new optical verification method is described. This method includes sounding of liquid flow with monochromatic coherent light, registration of impulse signals resulting from interaction of sounding radiation with cells, and measuring impulses' amplitude and duration and construction on the basis of obtained measurement results functions of two-dimensional pulses' amplitudes and duration distribution that express statistical intensity characteristics of light scattering by cells. On the basis of obtained functions the size distribution of cells is determined and apoptosis parameters are estimated. Results of apoptotic cells of K562 line (human leukemia), induced by cisplatm action, are described.