EFFECT OF GREEN TEA EXTRACT ON DNA DAMAGE AND CHROMOSOME ABERRATION DUE TO MULTIPLE ORGANIC POLLUTANTS IN TAP WATER

摘要

Organic pollutants have become widespread pollutants in the water and now represent a global contamination problem. In our present study, green tea extract (GTE) protect primary cultured rat hepatocytes (PCRH) against DNA damage induced by multiple organic pollutants (MOP) in tap water was test in comet assay, and inhibition the chromosome aberration induced by MOP using mouse micronucleus test. Meanwhile, the quantity of malondiadehyde (MDA) in the culture medium released by PRCH was measured. Results showed GTE could protect PRCH against DNA damage from MOP in a dose-dependent manner and inhibit the chromosome damage induced by MOP. One of the mechanisms of protection against DNA damage is inhibiting the oxidizing damage.