GENETIC DIVERSITY OF Brassica napus ACCESSIONS FROM CHINA AND CZECH REPUBLIC BASED ON RAPD MARKERS

摘要

The genetic diversity and the relationships among a collection of Brassica napus accessions, including 20 Chinese, 25 Czech, 2 Germany, 2 France, and 1 England were evaluated using random amplified polymorphic DNA (RAPD) markers. A total of 79 polymorphic amplified products were obtained from 10 decame primers. The Hamman metric based distance was chosen for which the best fit between a priori and a posteriori grouping was found using UPGMA. Cluster analysis based on Hamman genetic distance placed accessions in the experiment into three main groups; the first group included 16 rape accessions from China and the second group that from Czech with the exception of "Zhongshuang No.2", a Chinese winter rape. The third group included accessions both from China and Czech. There existed genetic variation among the experiment accessions, however, the genetic variation between Chinese accessions and Czech accessions was greater than that among Chinese accessions and that among Czech accessions. In view of the obvious genetic differences, the Czech B. napus accessions would be important resources for enriching the genetic background of Chinese rapeseed and vice versa.