IDENTIFICATION OF HUMAN METABOLITES OF 2,3,7,8-TCDD

摘要

For the first time, two polar metabolites of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were identified in various tissues of a human suffering from severe TCDD intoxication. 2,3,7-trichloro-8-hydroxydibenzo-p-dioxin and 1,3,7,8-tetrachloro-2-hydroxy-dibenzo-p-dioxin were identified in serum, faeces, and urine by gas chromatography high resolution mass spectrometry. Highest amounts of these metabolites were detected in faeces whereas serum and urine contained only traces. The relative amount of TCDD metabolites excreted via faeces accounts to approximately 50% of the totally eliminated TCDD by this route. As only small amounts of TCDD metabolites were detected in urine, renal excretion of these compounds can be considered as minor elimination pathway. In serum the relative amount of metabolites was 50 times lower than in faeces, probably due to the rapid elimination of the metabolites after formation in the liver by phase I and II enzymes and transferred via the bile to the intestine.