Imaging by Shape

摘要

Quantitative phase imaging generates the refractive and thickness structure of transparent samples in transmission light microscopy. It is a method that utilises the fact that a phase distribution in one plane has visible outcomes on the intensity of the wave as it propagates. This paper shows equivalent shape imaging results appear possible for through-focal stacks in reflection microscope imaging. Comparison is made to the transmission formulation and images to show the appropriateness of the results.