Rapid identification of the multidrug resistance in the human leukemic cells by near-infrared Fourier transform Raman microspectroscopy

摘要

Abstract: In this work, we have studied the cancer cell lines sharing a common feature: the multi-drug resistance where P- glycoprotein is responsible for the active efflux of the drug out of the cell. For this, we have used two types of cells, MDR-human leukemic K562 cells and non-MDR acute promyelocytic leukemic HL60 cells. The comparison between normalized micro FT-Raman spectra of resistant and sensitive K652 cells shows a decrease in the intensity of the amide I and III bands and a down shift of the amide I band. On the other hand, control experiments with HL60 cells do not show any remarkable changes. Analysis of micro-FT-Raman spectra by resolution enhancement methods and by chemometrics tools reveal further information concerning the conformational changes of the cell constituents involved in the expression of the MDR-phenotype. !16