Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion

摘要

A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes, adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic microscope. Quantitative phase images of live cellular specimens are presented.