Dynamic molecular imaging of living cells by using Raman scattering

摘要

Raman spectroscopy has been utilized to investigate properties of biomolecules due to its capability of detecting molecular vibrations that represent molecular species, structures and environmental conditions. In this research, we used Raman scattering to image dynamics of molecules in living cells. By combining the 532 nm excitation and a slitr scanning detection technique, we observed dynamics of molecular distributions of cytochrome, protein beta sheets, and lipids in an unstained HeLa cell during mitosis with a frame rate of 5 minutes.