Protein anchoring in silicon doped hydroxyapatite 3D-scaffolds via biotinylation

摘要

One of the challenges in bone tissue engineering is the anchoring of growth factors on the surface of 3D-scaffolds [1]. Protein immobilization remains a dispute to the life science industry, due to the so many requirements: (i) a sufficiently strong and specific affinity of proteins with the surface; (ii) site-directed immobilization; and (iii) preservation of a native conformation and biological function of proteins in their adsorbed stages. Several authors have developed methodologies of covalent protein immobilization after chemical modification of hydroxyapatite ceramic surface, through its functionalization with 3-