Titanium with Nanotopography Drives Mesenchymal Stem Cells to Osteoblast Differentiation through miR-4448, -4708 and -4773 Downregulation

摘要

The results showed that the Ti with nanotopography directs hMSCs differentiation toward the osteoblast lineage. In addition, we have identified 3 miRs that target SMADs and play critical role in the osteoinductive effect of this nanotopography on hMSCs. By downregulating miR-4448, -4708, and 4773, Ti with nanotopography attenuates SMAD1 and SMAD4 degradation, intensifying BMP-2 signal transduction, which stimulates osteoblast differentiation. This novel mechanism involving a miR-SMAD-BMP-2 network in the nanotopography-mediated osteoblast differentiation is a major step for developing Ti surface modifications to modulate the osseointegration process.