COMPARISON OF DIFFERENT METHODS OF PURIFICATION OF ENVIRONMENTAL SAMPLES FOR ANALYSIS OF POLYCYCLIC AROMATIC HYDROCARBONS

摘要

The Stockholm Convention on Persistent Organic Pollutants (POPs) was signed in May 2001 by 127 countries. Currently, 12 substances are regulated by the convention, and the work on finding new candidate chemicals to the convention has started. One group of substances in focus is polycyclic aromatic hydrocarbons (PAH). In this study, the development of a simple methodology for the determination of several PAHs, using the isotope dilution technique by GC/MS was performed. In this work, an assessment of a new automated clean-up system based on gel permeation chromatography (AccuPrep MPS(TM), J2 Scientific) combined with an in-line concentration system (AccuVap(TM), J2 Scientific) was performed. The system is used for the purification of a wide range of samples (pesticides, mycotoxins, antibiotics etc...) in different matrices such as foods, tissues, plants and environmental samples (soil, sludge, hazardous waste as described by the method EPA, then enlarging the purification of dioxins, polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). The suitability of the new automated system in the analysis of real samples was evaluated, by comparing chromatographic traces and values obtained by analyzing different rates of the same sample, purified by the methods in exam: Florisil, silica and GPC.