Abstract: Carious regions of human teeth were investigated by means of remission-, fluorescence excitation- and fluorescence emission spectroscopy as well as time-resolved fluorescence measurements in the ns region. A detection of dental caries is possible with all of these spectroscopic methods. The peaks in measured remission- and fluorescence excitation spectra of carious regions correspond with the absorption bands of metal free porphyrins. The emission spectra and fluorescence decay kinetics indicate that protoporphyrin IX may be the main chromophore. HPLC measurement verify the existence of proto- (main component), copro- and probably Zn-protoporphyrin.!11
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