Near Infrared Raman Spectra of Rhizoma Dioscoreae

摘要

A novel and compact near-infrared (NIR) Raman system is developed using 785-nm diode laser, volume-phase technology holographic system, and NIR intensified charge-coupled device (CCD). Raman spectra and first derivative spectra of Rhizoma Dioscoreae are obtained. Raman spectra of Rhizoma Dioscoreae showed three strong characteristic peaks at 477.4cm~(-1), 863.9cm~(-1), and 936.0cm~(-1).The major ingredients are protein, amino acid, starch, polysaccharides and so on, matched with the known basic biochemical composition of Rhizoma Dioscoreae. In the first derivative spectra of Rhizoma Dioscoreae, distinguishing characteristic peaks appeared at 467.674cm~(-1)、484.603cm~(-1)、 870.37cm~(-1)、 943.368cm~(-1).Contrasted with Rhizoma Dioscoreae Raman spectra, in 600cm~(-1) to 800cm~(-1), 1000cm~(-1) to 1400cm~(-1) regions, changes in Rhizoma Dioscoreae Raman first derivative spectra are represented more clearly than Rhizoma Dioscoreae Raman spectra. So Rhizoma Dioscoreae raman first derivative spectra can be an accurate supplementary analysis method to Rhizoma Dioscoreae Raman spectra.