Visualization of intracellular Ca~(2+) dynamics with Third Harmonic Generation microscopy

摘要

Measurements by Laser scanning Third Harmonic Generation microscopy of Ca~(2+) dynamic release from internal stores and/or calcium influx in biological cells is presented. The Ca~(2+) signal consists of a transient increase in the intracellular concentration. A good correlation is found between these measurements and measurements done with microspectrofluorometry.