Analysis of RBC Damage Using Laser Tweezers Raman Spectroscopy (LTRS) During Femtosecond Laser Optical Trapping

摘要

We monitored cell viability and damage under femtosecond laser irradiation using aser weezers Raman pectroscopy (LTRS) which is becoming a powerful tool for the analysis of biological materials. Femtosecond lasers are more frequently used as a light source for optical tweezers since they enable nonlinear optical phenomena such as two-photon absorption or second harmonic generation trapping. Femtosecond laser optical trapping similar to thee CW laser optical trapping except that optical damage can be easily induced due to extremely high peak power of femtosecond pulses. We monitored the Raman signal changes as a marker for optical damage. We used red blood cell (RBC) as a target sample and first used the CW laser beams to trap the RBC from the bottom of the chamber. After the trapped RBC is moved to a desired depth, we switched the laser mode to mode-locked mode and monitored the Raman signals as a function of the laser irradiation time. It was observed that the Raman shift at 1543 cm~(-1) may be a good marker for optical damage both for CW and femtosecond laser trapping.