Interferometric investigations of the cosmonaut erythrocytes

摘要

Many biological objects, include blood cells, are optically transparent. They don't absorb light and only change optical path length by variations of object's thickness of its refractive index variations. The phase shifting interferometry is used for the visualization of the phase information and the obtaining quantitative properties of cells. Cells are fixed by alutaraldehyde and mounted in immersion liquid for reduction the influences of refraction. Blood cell's interferogram contains the information about space distribution refractive index. The phase-shift method was used for its reconstruction. This method inclues record of four interferograms used different values of phase-shift of the reference wave. Many blood cells interferograms are obtained (lymphocytes and erythrocytes). The shape, size and density of cells are measurement. We suppose that these date will be ones of the early diagnostically criteria of hematological diseases. Experimental results for interferometric microscopy and tomography of blood cells are presented.