BIOSENSOR BASED ON CHEMILUMINESCENCE FOR SERUM URIC ACID DETERMINATION

摘要

A biosensor based on luminol chemiluminescent reaction and immobilized uricase column for uric acid determination has been developed. The response time by the sensor for various concentration of serum uric acid was 47 seconds with 17 μl sample volume and at 40 samples per hour. The linear range of standard curve was from 1 mg/dl up to 20 mg/dl of serum uric acid. The within day imprecision was 3.22-4.36%. The day to day imprecision was 6.18-7.80%. The recovery rate by the method was 93-109%. Compared with standard co-lorimetric method employed enzymatic kit revealed that the linear regression and correlation coefficient were Y=0.4+0.938X and r=0.9909 respectively. The immobilized uricase column retained 94% of its original activity even after over 2000 runs for five and half monthes' continual usage.