Development of a platform expression system using targeted integration in Chinese Hamster Ovary cells

摘要

Our team is building an improved CHO cell line that will enable the efficient and targeted integration of transgenes at a landing pad site that supports strong and staple expression. The use of targeted integration in upstream cell line development can reduce the heterogeneity of stable pools and deliver clones with more consistent expression and performance, shortening development timelines. We started by randomly integrating a mAb containing landing paid into CHO to screen for pools and clones capable of high expression from a tow copy number. Our workflow has yielded a number of clones with rrtAb expression >2g/L in fed batch assays. Stability studies are underway as well as genomic characterization of the integration sites in our top clones.