Folic Acid Conjugated Polymer as a New Active Tumor Targeting Drug Delivery Platform

摘要

The molecular modelling characterization and synthesis of the folate functionalized biomaterial poly(styrene-alt-maleic anhydride), PSMA predicted the folic acid functionalized SMA as a good candidate for targeted drug delivery. Folate was attached to the pH responsive SMA polymer via a biodegradable linker 2,4-diaminobutyric acid, DABA. The computational results predicted DABA as the optimal linker and showed that the functionazlied PSMA is linear at neutral condition and its linearity remains pH responsive. However, the earlier synthesis route displayed difficulties in characterization and purification due to the complexity of polymeric products. In this paper, a different synthesis route is shown along with NMR and IR characterization. We will show some preliminary results of applying biomaterials in cell lines. Curcumin, a natural ingredient was selected as a fluorecent marker to study polymer-cell binding efficiency as well as cellular uptake. The WST assy results confirmed that both SMA and FA-DABA-SMA polymer templates are not toxic to the cells and the delivrying vehicles proved to be effective in releasing hydrophobic anti-tumor drugs into the tumor cells.