Damage to cryopreserved cells is dependent on the kinetics and morphology of ice formation in supercooled thermodynamic states. To gain a better understanding of ice crystallization phenomena under such non-equilibrium conditions, we undertook an investigation of crystal growth in highly supercooled water droplets in the range of −28°C to −38°C. Microscale droplets of distilled water were frozen, and the ice crystal growth process was recorded using a high-speed imaging system. Crystal growth velocities were found to range from 245 mm/s to 470 mm/s, and were significantly lower than growth velocities predicted by the Langer Müller-Krumbhaar theory, suggesting a crossover from a diffusion-limited regime to a kinetic-limited regime at high supercooling.
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机译：冷冻保存细胞的损伤取决于过冷热力学状态下冰形成的动力学和形态。为了更好地了解在这种非平衡条件下的冰结晶现象，我们对-28°C至-38°C范围内的高度过冷水滴中的晶体生长进行了研究。冷冻蒸馏水的微滴，并使用高速成像系统记录冰晶的生长过程。发现晶体的生长速度在245 mm / s至470 mm / s的范围内，并且显着低于LangerMüller-Krumbhaar理论预测的生长速度，这暗示了从扩散受限机制到动力学受限机制的转变。高过冷度。
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