INVESTIGATION OF THE INFLUENCES OF MICRO VIBRATIONAL STIMULI AND HYDROPHILICITY OF A SCAFFOLD ON A BOVINE ENDOTHELIAL CELL CULTURE

摘要

Both the hydrophilicity of the scaffold and applied sheer stress can influence the growth of cultured cells. In this study, the influences of applied shear stress and the hydrophilicity of the scaffold on the growth of bovine endothelial cells (BEC) were investigated. A piezoelectric micro vibrational stage was used to provide micro vibrational stimuli of different frequencies to generate various sheer stresses. 24-well, biodegradable lactide-co-glycolide (PLGA) scaffolds, and nanostructuresd PLGA scaffolds were used for the cell culturing. From the results of this study, it can be inferred that the vibration induced sheer stress can effectively enhance BEC growth as long as the corresponding sheer force is less than the adhesive force between a cell and the scaffold. It is also suggested that micro vibration stimulus may be a more cost and time effective solution than the nanostructured scaffold approach for the enhancement of BEC growth.