Feasibility and strategy of high efficiency fermentative H2 production from corn stalk liginocellulose

摘要

In order to investigate the posibility of a 'pretreatment-delignification ?usimultaneous saccharification and fermentation (SSF)' strategy for bio-hydrogen from cellulosic feedstock, batch tests were conducted using co-culture of mesophilic isolates, Clostridium acetobutylicum X9 (could effectively hydrolyze the partially delignified lignocellulose complex) and Ethanoigenens harbinense B49 (highly hydrogen production bacterium). The results showed that for the pretreatment-delignification step, acidic stream explosion (1% w/v H2SO4, 121 ℃, 15 psi for 2h) was applied to pretreat the corn stalk, and the residue following steam explosion was washed by water to separate the lignocellulose solid. For the SSF step, C. acetobutylicum X9 and E. harbinense B49 were inoculated, and simultaneous the resulting hydrolysate was used as the carbon substrate for initial pH of 7.0 for 30 h. With an initial corn stalk concentration of 15 g/l, the H2 production and yield was approximately 458.0 ml/l and 6.3 mmol H2/g cellulose, and cellulose degradation ratio of 63.3% were obtained respectively. This work implied the feasibility of a 'delignification→usimultaneous saccharification and fermentation' process for hydrogen production from corn stalk liginocellulose.