Study on Properties and Structure Characterization of New Type Collagen Scaffold Materials

摘要

In order to overcome the shortcomings of acellular dermal matrix which is used as tissue engineering scaffold material, such as bad permeability, low degradation speed, strong immunogenicity and so on, a new type of collagen scaffolds were prepared by adopting various chemical, biological and physical comprehensive methods to deal with pigskin. The microstructure of the scaffolds was observed under photomicroscope and scanning electron microscopy (SEM) and the vitro degradation time, permeability, tensile-strength, porosity, shrinkage temperature were studied. Experimental results showed: the dermal fibroblasts, lipocytes and interstitial substances between collagen fibers had been removed completely, simultaneously collagen fibers had been appropriately loosen, and native dermal structure was retained; the permeability was 3000g·m~(-2)·24h~(-1)around, which was fit for wound healing; the degradation time was between 25h and 50h, and could be controlled by adjusting technological conditions; the tensile-strength was between 10.20MPa and 11.50MPa which was better; the shrinkage temperature was between 70℃ and 85℃. All of these indicated that the materials had overcome the shortcomings of acellular dermal matrix, and had high permeability, tensile strength, and proper degradation speeds. So it conformed to the tissue engineering scaffold material requirements.