The UV Shielding, Antimicrobial and Antifungal Effect of Novel Nano-SiO_2/Collagen Materials

摘要

A composition of collagen-SiO_2 organic-inorganic nano-hybrid was prepared by sol-gel process. FTIR analysis demonstrated that the highly active nano-SiO_2 particles produced by the hydrolysis of the precursor (tetraethyoxyl silane) formed chemical bonds of Si—C with—C=N—groups in arginine, histidine and trypophane on the backbone of collagen; In the meantime, as the condensation of silanol from the hydrolysis proceeded, Si—OH could react with the pendent hydroxyl group of the collagen and therefore, strong reaction occurred between organic and inorganic phases. The particle size of SiO_2 in protein was affected by the pH value and the concentration of SiO_2. When the SiO_2 concentration and the pH were suitable, the size of the nano-SiO_2 in collagen was controlled to a range from 80 to 90 run. Analysis from Atomic Force Microscopy (AFM) revealed that SiO_2 particles produced by hydrolysis of precursor were evenly distributed in the protein and no apparent conglomeration of SiO_2 was found in the final product. When SiO_2 concentration was high, the size of the inorganic particles was larger than 300 nm. Collagen modified with nano-SiO_2 showed a strong UV shielding effect in a range of wavelength from 200 to 250nm. Meanwhile the modified collagen had found to have a highly ability of antimicrobial and antifungal.