Combination of adenovirus and cross-linked low molecular weight PEI improves efficiency of gene transduction

摘要

Recombinant adenovirus mediated gene therapy is an exciting novel strategy in cancer treatment. However, poor infection efficiency on coxsackievirus and adenovirus receptor (CAR) down-regulated cancer cell lines is one of the major challenges for its practical and extensive application. In our study, adenovectors were complexed with a new synthetic polymer PEI-DEG-bis-NPC (PDN) based on polyethylenimine (PEI) 2k. The obtained Ad/PDN complexes could improve the performance of the adeno-vectors alone or Ad/PEI 25k complexes in both cultured cancer cell lines and in vivo tumor treatment.