Custom bile duct phantom for first-in-human multiplexed NIR fluorescence peptide imaging

摘要

Multimodal endoscopy, with fluorescence labeled peptides specific for multiple biomarkers, is a promising technique todetect early-stage GI tract cancers in vivo. A reproducible bile duct phantom with near infrared (NIR) fluorescent targetsis developed for practicing the clinical study protocol and quantitative evaluation of multimodal endoscope performanceduring biliary duct imaging. Furthermore, this phantom with strictures will be used for testing new fluorescence guidedbiopsy devices.Materials and Methods: A soft, flexible synthetic human bile duct was fabricated from a paintable silicone rubber. Dueto the complex structure of biliary system (such as hepatic ducts, cystic duct, and common bile duct), the template moldfor lumen was designed for 3D printing using Polyvinyl Acetate (PVA), which can be later dissolved in water. Curedgelatin patches with different concentrations of fluorescence dyes (Cy5 and IRDye800) were placed onto the mold. Thensilicone rubber with pigments to simulate visual appearance was painted in layers. After the silicone curing, the phantomwas placed in warm water (40 degreeC) to dissolve PVA. Two different multimodal scanning fiber endoscope systems,RGB reflectance + NIR fluorescence and 3 fluorescence (IRDye800, Cy5, and FITC) + grayscale reflectance, were usedto test the phantom.Results: The bile duct phantom is flexible, stable, and repeatable to fabricate with strictures. Clinical study procedures offluorescence labeling were evaluated quantitatively. The NIR fluorescence targets in the phantom were used to calibratethe imaging system, further develop image-based biomarker quantification.