Capillary array electrophoresis: an approach to high-speedhigh-throughput DNA sequencing,

摘要

Abstract: -excited, confocal-fluorescence scanner is used for high- sensitivity, on-column detection of electrophoresis performed using an array of capillaries. The capabilities of this method and apparatus are illustrated by application to high-speed, high- throughput DNA sequencing. Sanger DNA sequencing fragments are separated on an array of capillaries and then distinguished by using a binary coding scheme that employs only two different fluorescently labeled dye primers to identify four sets of fragments. DNA sequencing results are presented using a 25- capillary array. This apparatus has the capability of sequencing DNA at a rate of approximately 25,000 bases/hour.!22