Genome-Wide Transcriptional Analysis of Al-responsive Genes in Rice

摘要

Rice ( Oryza saliva) is the most Al-resistant crop among small-grain cereal crops, but the mechanism underlying its high Al resistance is still poorly understood. Here, we compared transcriptional profile of Al-responsive genes in the root tips and mature zones between the Al-tolerant cultivar ( Koshihikari) and a hyper-sensitive mutant star/als1 ( sensitive to Al rhizotoxicity 1) by using Agilent 44K Rice Oligo DNA Microarray. Exposure to 20 μM Al for 6 h caused increased expression ( higher than 3 folds) of 786 and 5547 genes including 624 common genes, respectively, in the root tips of WT and the mutant. On the other hand, in the mature root zone, genes up-regulated by Al were 616 and 8676 including 453 common genes, respectively, in the WT and mutant. From these data, putative Al-tolerance and toxicity genes were extracted by comparing gene expression between the WT and the mutant.