The experimental study on culturing Viruses in environmental water with the method of L20B cell absorption

摘要

Objective To set up a simple, practical, new method for detecting minimum viruses in large volume water. Methods Adding L20B cells into the large volume water containing minimum viruses. Collecting the cells after they fully reacted with viruses, then culturing vuruses, with the method of NaCl--AlCl_3 sedimentation as control. Results If the amount of viruses was 100, 10TCID_50 per 1000ml water, the viruses could be detected. When the amount was 1 approx 2 TCID_50, the viruses in 13 of 14 samples could be detected. While the viruses, of 100TCID_50 per 1000ml water in 1 of 4 samples, could be detected with the method of NaCl--AlCl_3 sedimentation, but hte viruses of other concentrations in all samples couldn't be detected.