2D KINETICS OF SURFACE-BOUND P-SELECTIN/LIGAND INTERACTIONS

摘要

Kinetics of P-selectin binding to carbohydrate ligands was measured using a micropipet aspiration assay. Reaction rates and binding affinity were estimated from the experimental data using a small system probabilistic model. This work can be extended to analyze kinetics of receptor-ligand binding in inflammatory process, such as rolling of neutrophils on endothelium.