DNA Array with nanoparticle probes for the detection of P53 mutations of Lung Cancer

摘要

The tumor suppressor gene p53 is the gene that most commonly becomes mutated in lung cancer. A gold nanoparticle probe-based array assay has been developed for direct detection of the p53 mutation in clinical lung cancer samples with high efficiency. The morphologic essential were three-component, 'sandwich' hybridization of unlabelled target and nanoparticle-oligonucleotide conjugate probe, followed by amplification with signals of gold nanoparticles using silver staining enhancement. This method enables discrimination single base changes in total genomic DNA without target or signal amplification. In comparison to a previously reported fluorescence-based method, this method remarkably increased detection sensitivity and specificity, and the sandwich colorimetric assay can be detected by a CCD camera or a commonly optical scanner. We have applied this method to the rapid and sensitive distinguished "hot spots" from the p53 genes in lung cancer from wild type.