PRODUCTION OF ALKALI-TOLERANT ENDOGLUCANASE BY HOMOLOGOUS OVEREXPRESSIO OF GENE EGLS IN BACILLUS SUBTILIS-Y106 AND APPLICATION IN IMPROVEMENT OF PULP PROPERTIES

摘要

An endoglucanase gene eglS,was homologous overexpressed in Bacillus subtilis-Y106 to enhance the endoglucanase production.The resulting CMCase activity of crude enzyme from the liquor fermentation of the engineered strain was about 10.2 IU/mL at 36 h of fermentation,which was 10 times higher than that of the native Bacillus subtilis-Y106.Molecular weight,optimal temperature and pH of the endoglucanase EglS were detected respectively.The enzyme activity of 85％could be maintained at pH 8.5.Potential of the endoglucanase EglS in improvement of wheat straw pulp was also evaluated.