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Correction of distortion in Confocal Scanning Microscopy

机译:共聚焦扫描显微镜中畸变的校正

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摘要

In using beam scanning microscope with scanners as acousto-optic deflectors, there is a 'speed down' in vicinity of maximum deflected angle in spite of linear input as driving voltage, that is, there is some non-uniform scanning velocity on lateral direction. It results from non-linearity in AO scanning. By driving the calculated voltage from a constant angular velocity equation, the scanning range can be expanded. To overcome this DC offset of the image extinction that mainly results from AO diffraction and optical alignment error, the filtering is done about cut off frequency obtained in experiment that is carried out with optical mirror.
机译:在使用带有扫描仪作为声光偏转器的束扫描显微镜时,尽管线性输入作为驱动电压,但在最大偏转角附近仍存在“速度下降”,即,横向方向上存在一些不均匀的扫描速度。这是由于AO扫描中的非线性导致的。通过从恒定角速度方程驱动计算出的电压,可以扩大扫描范围。为了克服主要由AO衍射和光学对准误差导致的图像消光的DC偏移,对通过光学镜进行的实验中获得的截止频率进行了滤波。

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