PRESSURIZED FLUID EXTRACTION OF BIOACTIVE NUTRACEUTICALS FROM MARINE BIOMASS

摘要

Various species of marine macroalgae contain bioactive compounds including carotenoids, proteinsrnand polysaccharides. Fucoxanthin, the main carotenoid component, is known to possess numerous therapeuticrnfunctions. Similarly, low-molecular-weight fucoidan and peptides also exhibit several functions including antioxidantrnactivities, antitumor and anti-aging effects. This paper highlights the use of supercritical carbon dioxide (SCCO_2) andrnsubcritical water, an environmentally benign solvent for extraction of these useful compounds. Results indicated thatrnSCCO_2 extraction could recover the lipids obtaining fucoxanthin with a recovery close to 80%. We also found thatrncrude extract was more potent than pure fucoxanthin against HepG2 (liver) cancer cells. Subcritical water treatmentrnwas also performed using the extraction residues obtaining the target LMW fucoidan of 5-30 kDa even at a lowrntemperature of 140 ℃. The use of semi-batch reactor for simultaneous fractionation and separation of targetrnmolecular weights was also investigated. The advantages of coupling the process with microwave irradiation is alsornintroduced and discussed.